Pure nitrocellulose exhibits the highest sensitivity with very low backgrounds in all transfers, especially in protein blotting. Unlike PVDF, nitrocellulose wets out naturally, does not require methanol and will not turn hydrophobic during the transfer process. Easily blocked, nitrocellulose does not need the many blocking steps required with PVDF. Excellent results with all detection systems: antibody/antigen, radiolabeled, biotinylated and chemiluminescent for extreme flexibility in procedure design
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